Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Tosoh Corporation 是一家日本化学和特种产品及材料集团的母公司，该集团在全球拥有 100 多家公司，拥有超过 12,000 名多民族员工。 

母公司成立于1935年，在东京证券交易所第一部上市。在我们开展业务的 80 年中，我们建立了平衡的工业大宗化学品产品线以及高科技和利基市场的特种产品和材料产品线。

Tosoh 的主要市场包括化学和石化、建筑、汽车、消费电子、信息技术、生物科学和环境市场。 、

1935年

• 成立东洋苏打制作所（日文罗马字：Toyo Soda Kogyo Kabushikigaisha）

1964年

• 开设纽约办事处（Tosoh USA, Inc. 的前身，成立于 1979 年）

1968年

• 新大协和株式会社成立

1971年

• 四日市工厂开始运营

1975年

• 铁光社株式会社并入东洋苏打制作所

• 在希腊的Tosoh Hellas AIC（原 Tekkosha Hellas SA）开始生产电解二氧化锰

1976年

• 开始在印度尼西亚生产 PVC（PT Standard Toyo Polymer）

• 在阿姆斯特丹成立 Amto International 贸易公司（后于1987 年更名为Tosoh Europe BV）

1978年

• 荷兰的乙胺生产 ( Delamine BV ) 开始

• 新大协和中部化学株式会社合并，收购苯乙烯单体

• 植物

1979年

• Tosoh USA, Inc.成立

简而言之，Tosoh 是一家全球性的化工公司，为制造商提供他们所需的材料，以生产使现代生活变得丰富多彩的事物。